Detailed view for TGME49_231480

Basic information

TDR Targets ID: 259124
Toxoplasma gondii, GCN1, putative
Source Database / ID:  ToxoDB 

pI: 7.0191 | Length (AA): 3416 | MW (Da): 362621 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02985   HEAT repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0033674   positive regulation of kinase activity  
GO:0033554   cellular response to stress  
GO:0019901   protein kinase binding  
GO:0006417   regulation of translation  
GO:0043022   ribosome binding  
GO:0019887   protein kinase regulator activity  
GO:0005515   protein binding  
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1156 1273 5hmo (A) 810 926 12.00 0.13 0 0.157743 -0.29
1714 2665 5me3 (B) 444 1434 12.00 0 1 0.254289 0.79
1872 2066 4zv6 (A) 85 242 33.00 0.11 0.98 0.241684 0
1934 2709 3ea5 (D) 3 851 15.00 0 1 0.278766 0.53
2038 2873 5hdt (B) 255 1109 13.00 0 0.97 0.320331 0.54
2056 2259 2qk2 (A) 279 480 18.00 0 0.72 0.363319 -1.17
2477 2602 5ife (C) 594 731 27.00 0.74 0.99 0.353485 -1.01
3183 3352 2jdq (A) 336 507 13.00 0.0014 0.06 0.152966 -0.03
3187 3413 4rzp (A) 8 224 14.00 0.08 0.99 0.265652 -0.56

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_128125)

Species Accession Gene Product
Arabidopsis thaliana AT1G64790   protein ILITYHIA
Babesia bovis BBOV_II003920   HEAT repeat family protein
Brugia malayi Bm1_44525   HsGCN1
Candida albicans CaO19.5328   N terminus is frameshifted into CaP19.5333 upstream
Candida albicans CaO19.12793   similar to N terminus of S. cerevisiae GCN1 (YGL195W) translational activator of GCN4 via activation of eIF2alpha kinase GCN2 in
Candida albicans CaO19.12788   N terminus is frameshifted into CaP19.12793 upstream
Candida albicans CaO19.5333   similar to N terminus of S. cerevisiae GCN1 (YGL195W) translational activator of GCN4 via activation of eIF2alpha kinase GCN2 in
Caenorhabditis elegans CELE_Y48G9A.3   Protein GCN-1
Cryptosporidium hominis Chro.80225   hypothetical protein
Cryptosporidium parvum cgd8_1930   large protein with a GCN1 domain
Dictyostelium discoideum DDB_G0279487   GCN1-like protein
Drosophila melanogaster Dmel_CG17514   CG17514 gene product from transcript CG17514-RA
Echinococcus granulosus EgrG_000967600   translational activator gcn1
Entamoeba histolytica EHI_078260   HEAT repeat domain containing protein
Echinococcus multilocularis EmuJ_000967600   translational activator gcn1
Homo sapiens ENSG00000089154   GCN1 general control of amino-acid synthesis 1-like 1 (yeast)
Leishmania braziliensis LbrM.18.0910   hypothetical protein, conserved
Leishmania donovani LdBPK_180820.1   hypothetical protein, conserved
Leishmania infantum LinJ.18.0820   hypothetical protein, conserved
Leishmania major LmjF.18.0820   hypothetical protein, conserved
Leishmania mexicana LmxM.18.0820   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02023   hypothetical protein
Mus musculus ENSMUSG00000041638   GCN1 general control of amino-acid synthesis 1-like 1 (yeast)
Neospora caninum NCLIV_031730   translational activator, putative
Oryza sativa 4333935   Os03g0721200
Saccharomyces cerevisiae YGL195W   Gcn1p
Schistosoma japonicum Sjp_0111150   expressed protein
Schistosoma japonicum Sjp_0124290   IPR000357,HEAT,domain-containing
Schistosoma japonicum Sjp_0007570   Translational activator GCN1, putative
Schistosoma mansoni Smp_134860   translational activator gcn1-related
Schistosoma mansoni Smp_075950   hypothetical protein
Schmidtea mediterranea mk4.006229.01   Translational activator GCN1
Schmidtea mediterranea mk4.006229.00   Translational activator GCN1
Schmidtea mediterranea mk4.012988.00  
Trypanosoma brucei gambiense Tbg972.10.16050   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.13280   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_10_11380   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_10_11370   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508891.130   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503681.9   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506357.130   hypothetical protein, conserved
Toxoplasma gondii TGME49_231480   GCN1, putative
Trichomonas vaginalis TVAG_458760   translational activator gcn1, putative
Trichomonas vaginalis TVAG_417700   translational activator gcn1, putative

Essentiality

TGME49_231480 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.13280 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.13280 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.13280 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.13280 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_231480 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_231480 (Toxoplasma gondii), GCN1, putative
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